HPLC Technique Troubleshooting

HPLC Technique Troubleshooting

Chromatography is a versatile separation technique developed way back in 1903 by Mikhail Tsvet, a Russian botanist for the separation of plant pigments. Since then this technique has evolved as a powerful tool in the lab for the separation and identification of different compounds in a mixture.

Chromatography is used as an indispensable tool nowadays, with a wide range of applications In the:

  • Food Industry – For Separation and analysis of additives, preservatives, vitamins, proteins, Toxins & Nutritional value of foods
  • Pharmaceutical Industry – To purify materials, separate compounds, in drug discovery, etc.
  • Chemical Industry – For testing purity of air & water samples: look out for contaminants
  • Forensic Testing 
  • Field Of Molecular Biology

Types of chromatography

  • Column chromatography
  • Ion-exchange chromatography
  • Gel-permeation (molecular sieve) chromatography
  • Affinity chromatography
  • Paper chromatography
  • Thin-layer chromatography
  • Gas chromatography
  • Dye-ligand chromatography
  • Hydrophobic interaction chromatography
  • Pseudoaffinity chromatography
  • High-pressure liquid chromatography (HPLC)

It is a must-know technique for all researchers owing to its vast no of applications & Usage in multiple domains of research. Out of various chromatographic techniques, HPLC – High-Performance Liquid Chromatography Technique is the most important & widely used.

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